3S8W
D2 domain of human IFNAR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 82.660, 82.660, 225.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.925 - 2.600 |
| Rwork | 0.218 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.925 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 26344 | |
| <I/σ(I)> | 16.3 | 2.4 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 31% (w/v) PEG 4000, 200 mM Li2SO4, 100 mM Tris pH 8.5, 5% (v/v) MPD, 10 mM Na acetate, 1.5% (w/v) PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
