3S8S

Crystal structure of the RRM domain of human SETD1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97941
Spacegroup name	P 1 21 1
Unit cell lengths	35.650, 31.510, 39.320
Unit cell angles	90.00, 114.12, 90.00

Refinement procedure

Resolution	31.290 - 1.300
R-factor	0.17
Rwork	0.168
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	composite of pdb entries 3LPY and 2J8A
RMSD bond length	0.014
RMSD bond angle	1.425
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.290		1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.066	0.030	0.705
Number of reflections	19651	238	1410
<I/σ(I)>	12.39	32.5	1.9
Completeness [%]	98.9	96	96.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	1.5 M ammonium sulfate, 0.1 M bistris propane, pH 7.0, vapor diffusion, temperature 291K