3S8M
The Crystal Structure of FabV
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97902, 0.97930 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.520, 74.530, 107.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 1.600 |
| R-factor | 0.1859 |
| Rwork | 0.185 |
| R-free | 0.20510 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.890 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.184 |
| Number of reflections | 53994 | |
| <I/σ(I)> | 64.1 | 14.3 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 100 mM Bis-Tris, 200 mM NaCl, 1 mM DTT and 15% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
