3S84
Dimeric apoA-IV
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794947, 0.9796317, 0.9494888 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.825, 70.825, 512.383 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.860 - 2.400 |
| R-factor | 0.2016 |
| Rwork | 0.200 |
| R-free | 0.22910 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CRANK |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.860 |
| High resolution limit [Å] | 2.390 |
| Number of reflections | 31511 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch plate | 7.5 | 293 | Crystal grew in pH 8.5 PEG and AmSO4, Dehydration in 60% PEG, Batch plate, temperature 293K |
