3S83
EAL domain of phosphodiesterase PdeA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.193, 46.963, 63.770 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 39.910 - 1.340 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.19816 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.648 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.910 | 1.360 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.083 | 0.370 |
| Number of reflections | 48723 | |
| <I/σ(I)> | 28.3 | 2.8 |
| Completeness [%] | 97.1 | 90 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Bis Tris propane, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
