3S7E
Crystal structure of Ara h 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-25 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5718 |
| Spacegroup name | H 3 |
| Unit cell lengths | 93.368, 93.368, 237.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.690 - 2.710 |
| R-factor | 0.2376 |
| Rwork | 0.236 |
| R-free | 0.25968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ea7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.053 | 0.159 |
| Number of reflections | 19169 | |
| <I/σ(I)> | 18.5 | 6.6 |
| Completeness [%] | 92.1 | 64.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 15% PEG400, 100 mM NaCl, Na citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
