3S67
Crystal structure of V57P mutant of human cystatin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 140.135, 140.135, 140.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.480 - 2.260 |
| R-factor | 0.18869 |
| Rwork | 0.187 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g96 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.657 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.088 | |
| Number of reflections | 11234 | |
| <I/σ(I)> | 36.5 | 2.16 |
| Completeness [%] | 99.8 | |
| Redundancy | 14 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Imidazole pH=6.5 0.9M Sodium acetate, additive 2.0M NDSB-221, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
