3S61
Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 128.162, 128.162, 316.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.260 - 3.030 |
| R-factor | 0.22139 |
| Rwork | 0.216 |
| R-free | 0.27183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Oxidized form of PvdA |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.819 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 3.190 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Number of reflections | 26002 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 96.0 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 9.5% PEG 8000, 25% glycerol, 60 mM potassium phosphate monobasic, Reducing agent Sodium Dithionite, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
