3S60
Structure of the cyanobacterial Oscillatoria Agardhii Agglutinin (OAA) in free state obtained at 25 degree Celsius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-10-01 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.467, 40.658, 70.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.610 - 1.600 |
| R-factor | 0.18706 |
| Rwork | 0.182 |
| R-free | 0.22793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s5v |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.933 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.610 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.081 | 0.309 |
| Number of reflections | 13272 | |
| <I/σ(I)> | 8.7 | 1.5 |
| Completeness [%] | 95.0 | 91.5 |
| Redundancy | 2.48 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2.0 M (NH4)SO4 and 0.1 M Tris-HCl (pH 8.5), VAPOR DIFFUSION, SITTING DROP, temperature 298K |
