3S60
Structure of the cyanobacterial Oscillatoria Agardhii Agglutinin (OAA) in free state obtained at 25 degree Celsius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2010-10-01 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.467, 40.658, 70.374 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.610 - 1.600 |
R-factor | 0.18706 |
Rwork | 0.182 |
R-free | 0.22793 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s5v |
RMSD bond length | 0.022 |
RMSD bond angle | 1.933 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.610 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.081 | 0.309 |
Number of reflections | 13272 | |
<I/σ(I)> | 8.7 | 1.5 |
Completeness [%] | 95.0 | 91.5 |
Redundancy | 2.48 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2.0 M (NH4)SO4 and 0.1 M Tris-HCl (pH 8.5), VAPOR DIFFUSION, SITTING DROP, temperature 298K |