3S5V
Structure of the cyanobacterial Oscillatoria Agardhii Agglutinin (OAA) in free state obtained at -180 degrees Celsius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.899, 40.096, 68.986 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.670 - 1.550 |
| R-factor | 0.18165 |
| Rwork | 0.181 |
| R-free | 0.19302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3obl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.670 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.024 | 0.083 |
| Number of reflections | 15223 | |
| <I/σ(I)> | 52.7 | 7.1 |
| Completeness [%] | 98.7 | 89.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2.0 M (NH4)SO4 and 0.1 M Tris-HCl (pH 8.5), VAPOR DIFFUSION, SITTING DROP, temperature 298K |
