3S5U
Crystal structure of CRISPR associated protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9772 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.112, 140.091, 148.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.2187 |
| Rwork | 0.219 |
| R-free | 0.27530 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 59513 | |
| Completeness [%] | 99.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 0.1M MES, 0.1M Ca-acetate, 6-14% PEG6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
