3S5P

Crystal structure of ribose-5-phosphate isomerase B RpiB from Giardia lamblia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	P 31 2 1
Unit cell lengths	89.200, 89.200, 68.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.620 - 2.300
R-factor	0.2
Rwork	0.198
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vvr
RMSD bond length	0.014
RMSD bond angle	1.416
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.037	0.016	0.535
Number of reflections	13930	157	1003
<I/σ(I)>	22.39	60.4	2.53
Completeness [%]	97.6	84	96.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	289	GilaA.01163.a.A1 PS00138 at 24 mg/mL with 5 mM ribose-5-phosphate against JCSG+ screen condition A6 0.2 M Li2SO4, 0.1 M phosphate/citrate pH 4.2, 20% PEG 1000, crystal tracking ID 215117a6, VAPOR DIFFUSION, SITTING DROP, temperature 289K