3S5K
Crystal structures of falcilysin, a M16 metalloprotease from the malaria parasite Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.780, 105.290, 114.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.830 - 3.200 |
| R-factor | 0.216 |
| Rwork | 0.210 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fge |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.260 | 0.580 |
| Number of reflections | 36165 | |
| <I/σ(I)> | 3.3 | 1.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 20% PEG6000, 0.1M MES, 5 M Zn acetate,0.1% - mercaptoethanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
