3S51
Structure of FANCI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 114.700, 136.500, 149.700 |
| Unit cell angles | 115.90, 106.00, 95.00 |
Refinement procedure
| Resolution | 39.820 - 3.300 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.27780 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.869 |
| Data reduction software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.056 | 0.554 |
| Number of reflections | 110174 | |
| <I/σ(I)> | 23.1 | 1.4 |
| Completeness [%] | 95.9 | 80.3 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 40 mM tri-potassium citrate, 10 % (w/v) polyethylene glycol (PEG) 3350, 10 mM EDTA, and 10 mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
