3S4E
Crystal Structrue of a Novel Mitogen-activated Protein Kinase Phosphatase, SKRP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.852, 50.313, 57.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.970 - 1.260 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.18140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zzw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.280 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.046 | 0.091 |
| Number of reflections | 487871 | |
| <I/σ(I)> | 64.081 | 31.945 |
| Completeness [%] | 95.7 | 100 |
| Redundancy | 13.5 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 2.0M ammonium sulfate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
