3S2Y

Crystal structure of a chromate/uranium reductase from Gluconacetobacter hansenii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	119.677, 90.051, 95.234
Unit cell angles	90.00, 119.61, 90.00

Refinement procedure

Resolution	45.822 - 2.244
R-factor	0.1958
Rwork	0.193
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtt
RMSD bond length	0.008
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
Number of reflections	41698
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	20% PEG4000, 10% isopropanol, hepes 7.5, VAPOR DIFFUSION, temperature 293K