3S2Y
Crystal structure of a chromate/uranium reductase from Gluconacetobacter hansenii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.677, 90.051, 95.234 |
| Unit cell angles | 90.00, 119.61, 90.00 |
Refinement procedure
| Resolution | 45.822 - 2.244 |
| R-factor | 0.1958 |
| Rwork | 0.193 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| Number of reflections | 41698 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 20% PEG4000, 10% isopropanol, hepes 7.5, VAPOR DIFFUSION, temperature 293K |
