3S2X
Structure of acetyl-Coenzyme A synthase Alpha subunit C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-01-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 |
| Unit cell lengths | 129.040, 129.040, 46.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.600 - 2.350 |
| Rwork | 0.221 |
| R-free | 0.26360 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.078 | 0.456 |
| Number of reflections | 11621 | |
| <I/σ(I)> | 55.3 | 5 |
| Completeness [%] | 97.4 | 83.2 |
| Redundancy | 10.4 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 298 | 15% ethanol, pH 5.5, EVAPORATION, temperature 298K |
