3S28
The crystal structure of sucrose synthase-1 in complex with a breakdown product of the UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 276.750, 261.880, 160.190 |
Unit cell angles | 90.00, 108.70, 90.00 |
Refinement procedure
Resolution | 24.976 - 2.800 |
R-factor | 0.1903 |
Rwork | 0.188 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s27 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.139 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHENIX (1.7_650) |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 185.269 | 50.058 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.035 | 0.615 | |
Number of reflections | 263585 | ||
<I/σ(I)> | 9.1 | 13.3 | 1.3 |
Completeness [%] | 99.7 | 97.4 | 100 |
Redundancy | 3 | 2.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 1.85-1.90 M AMMONIUM SULFATE, 80-100 MM SODIUM CITRATE pH 6.1, 0.15 M SODIUM POTASSIUM TARTRATE, 150 mM SUCROSE, 5 mM URIDINE DIPHOSPHATE GLUCOSE, vapor diffusion, sitting drop, temperature 293K |