3S27
The crystal structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications.
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 276.212, 263.704, 159.663 |
| Unit cell angles | 90.00, 108.80, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.910 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.136 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.180 | 0.719 |
| Number of reflections | 232828 | |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 99.1 | 89.7 |
| Redundancy | 7.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 293 | 1.85-1.90 M AMMONIUM SULFATE, 80-100 MM SODIUM CITRATE pH 5.8, 0.15 M SODIUM POTASSIUM TARTRATE, 20 mM FRUCTOSE, 5 mM URIDINE DIPHOSPHATE, 2 mM DITHIOTHREITOL, vapor diffusion, sitting drop, temperature 293K |
