3S0T
Crystal structure of the CofA Type IV pilin subunit from enterotoxigenic E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-24 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.500, 34.150, 64.230 |
| Unit cell angles | 95.75, 97.38, 94.00 |
Refinement procedure
| Resolution | 25.000 - 1.260 |
| R-factor | 0.14151 |
| Rwork | 0.139 |
| R-free | 0.18065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oqv |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.200 | 1.310 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.067 | 0.501 |
| Number of reflections | 66018 | |
| <I/σ(I)> | 33.2 | 3.7 |
| Completeness [%] | 96.0 | 91.8 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2.5 M ammonium sulfate, 4.6 uM dodecyltrimethylammonium chloride, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
