3S01

Crystal structure of a Heterogeneous nuclear ribonucleoprotein L (Hnrpl) from Mus musculus at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97938
Spacegroup name	P 1 21 1
Unit cell lengths	48.672, 37.964, 57.953
Unit cell angles	90.00, 105.44, 90.00

Refinement procedure

Resolution	29.512 - 2.150
R-factor	0.1607
Rwork	0.159
R-free	0.20560
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.512	29.512	2.230
High resolution limit [Å]	2.150	4.610	2.150
Rmerge	0.091	0.040	0.452
Number of reflections	11314	2137	2242
<I/σ(I)>	6.8	16.1	1.9
Completeness [%]	98.1	95.7	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	10.00% 2-propanol, 20.00% polyethylene glycol 4000, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K