3S01
Crystal structure of a Heterogeneous nuclear ribonucleoprotein L (Hnrpl) from Mus musculus at 2.15 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-10 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91162,0.97938 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.672, 37.964, 57.953 |
Unit cell angles | 90.00, 105.44, 90.00 |
Refinement procedure
Resolution | 29.512 - 2.150 |
R-factor | 0.1607 |
Rwork | 0.159 |
R-free | 0.20560 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.512 | 29.512 | 2.230 |
High resolution limit [Å] | 2.150 | 4.610 | 2.150 |
Rmerge | 0.091 | 0.040 | 0.452 |
Number of reflections | 11314 | 2137 | 2242 |
<I/σ(I)> | 6.8 | 16.1 | 1.9 |
Completeness [%] | 98.1 | 95.7 | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 10.00% 2-propanol, 20.00% polyethylene glycol 4000, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |