3RZY

Human adipocyte lipid-binding protein FABP4, Apo form at 1.08 Ang resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.94312
Spacegroup name	P 21 21 21
Unit cell lengths	32.525, 53.423, 74.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.500 - 1.080
R-factor	0.15437
Rwork	0.152
R-free	0.18914
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	2.172
Data scaling software	SCALA (3.3.16)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.500	30.680	1.130
High resolution limit [Å]	1.080	3.400	1.080
Rmerge		0.037	0.437
Number of reflections	53794
<I/σ(I)>	11.7	15.6	1.7
Completeness [%]	93.9	89.1	80.7
Redundancy	4	4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	1.6 M sodium citrate, pH 6.5, vapor diffusion, temperature 293K