3RZ9
Mouse importin alpha-Ku80 NLS peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.43 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.357, 88.295, 98.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.490 - 2.290 |
| R-factor | 0.17952 |
| Rwork | 0.177 |
| R-free | 0.21797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.984 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.490 | 2.349 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 27469 | |
| Completeness [%] | 98.7 | 98.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
