3RYR
Domain-domain flexibility leads to allostery within the camp receptor protein (CRP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 85 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-21 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.570, 101.280, 54.120 |
| Unit cell angles | 90.00, 111.12, 90.00 |
Refinement procedure
| Resolution | 35.750 - 2.700 |
| R-factor | 0.20542 |
| Rwork | 0.197 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1il5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.099 | 0.346 |
| Number of reflections | 11687 | |
| <I/σ(I)> | 10.4 | 3.5 |
| Completeness [%] | 92.4 | 94.6 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% ETHANOL, 100MM PHOSPHATE, PH 6.8, 3MM CAMP, 5% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, VAPOR DIFFUSION, HANGING DROP |
