3RY2
Wild-type core streptavidin-biotin complex at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.426, 94.062, 104.608 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.950 |
| R-factor | 0.1161 |
| Rwork | 0.115 |
| R-free | 0.13130 |
| Structure solution method | isomorphous to previous structure |
| Starting model (for MR) | 1mk5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.761 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Fc (calculation) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.010 | 50.000 | 0.900 |
| High resolution limit [Å] | 0.880 | 2.390 | 0.880 |
| Rmerge | 0.049 | 0.032 | |
| Number of reflections | 150014 | ||
| <I/σ(I)> | 49.14 | ||
| Completeness [%] | 83.4 | 99.4 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 30% saturated ammonium sulfate, 0.1 M sodium acetate, 0.2 M sodium chloride, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
