3RY1
Wild-type core streptavidin at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.018, 84.432, 45.994 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 47.460 - 1.030 |
| R-factor | 0.1178 |
| Rwork | 0.117 |
| R-free | 0.13500 |
| Structure solution method | Isomorphous to previous structure |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.818 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Fc (calculation) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.460 | 4.000 | 1.050 |
| High resolution limit [Å] | 1.030 | 2.552 | 1.030 |
| Rmerge | 0.037 | 0.028 | 0.222 |
| Number of reflections | 206004 | ||
| <I/σ(I)> | 27.227 | ||
| Completeness [%] | 97.4 | 77.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 52% MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
