3RWO
Crystal structure of YPT32 in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.000, 45.200, 73.400 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 68.750 - 1.700 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cpj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.750 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 | 0.401 |
| Number of reflections | 44104 | |
| <I/σ(I)> | 3.9 | |
| Completeness [%] | 99.4 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, MGCL2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
