3RWB
Crystal structure of complex of 4PAL (4-Pyridoxolactone) and PLDH (tetrameric pyridoxal 4-dehydrogenase) from Mesorhizobium loti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-09 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.537, 50.211, 94.266 |
| Unit cell angles | 90.00, 90.47, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.699 |
| R-factor | 0.1365 |
| Rwork | 0.135 |
| R-free | 0.16620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nug |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.192 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.066 |
| High resolution limit [Å] | 1.699 |
| Number of reflections | 88415 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M Sodium acetate, 0.1M Tris-HCl, PEG 4000 30%, 0.65mM Pyridoxal, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
