3RW6
Structure of nuclear RNA export factor TAP bound to CTE RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.412, 117.048, 84.773 |
| Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
| Resolution | 37.866 - 2.300 |
| R-factor | 0.2059 |
| Rwork | 0.203 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3RW7 and an idealized A-form RNA 7-nt duplex |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.338 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.074 | 0.063 | 0.231 |
| Number of reflections | 44640 | ||
| <I/σ(I)> | 17.7 | ||
| Completeness [%] | 96.6 | 97.8 | 92.3 |
| Redundancy | 4.8 | 6.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 2 M ammonium acetate, 0.1 M Na acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
