3RW6

Structure of nuclear RNA export factor TAP bound to CTE RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	55.412, 117.048, 84.773
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	37.866 - 2.300
R-factor	0.2059
Rwork	0.203
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3RW7 and an idealized A-form RNA 7-nt duplex
RMSD bond length	0.007
RMSD bond angle	1.338
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.074	0.063	0.231
Number of reflections	44640
<I/σ(I)>	17.7
Completeness [%]	96.6	97.8	92.3
Redundancy	4.8	6.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	2 M ammonium acetate, 0.1 M Na acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K