3RVZ
Crystal structure of the NavAb voltage-gated sodium channel (Ile217Cys, 2.8 A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.005 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 126.277, 126.143, 192.444 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.2671 |
| Rwork | 0.267 |
| R-free | 0.29220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | built using SAD data sets from Hg and SeMet crystals |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.052 | 0.346 |
| Number of reflections | 31615 | |
| <I/σ(I)> | 20.61 | 2.08 |
| Completeness [%] | 82.9 | 59.3 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
