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3RVS

Structure of the CheYN59D/E89R Tungstate complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]100
Detector technologyCCD
Collection date2011-03-27
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1.02631
Spacegroup nameP 21 21 21
Unit cell lengths53.560, 53.620, 162.820
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.306 - 2.100
R-factor0.1498
Rwork0.148
R-free0.18370
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1fqw
RMSD bond length0.010
RMSD bond angle1.328
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.7_650))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.150
High resolution limit [Å]2.1002.100
Number of reflections52812
<I/σ(I)>15.324.33
Completeness [%]99.9100
Redundancy11.211.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52982.6 M Ammonium Sulfate, 100 mM Tris, pH 7.5, 5% (v/v) glycerol, 10 mM Sodium Tungstate, 20 mM Manganese chloride, 4.25 mg/mL CheY, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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