3RVC

Effector domain of NS1 from influenza A/PR/8/34 containing a W187A mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-05
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	31.992, 102.773, 67.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 1.800
R-factor	0.1999
Rwork	0.195
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.098	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	9857
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M Bis Tris, 0.2M NaCl, 22% PEG 3350, 20mM Xylitol, 40mM thieno[2.3-b]pyridin-2-ylmethanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K