3RUI

Crystal structure of Atg7C-Atg8 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796, 0.9799, 0.95
Spacegroup name	P 43 21 2
Unit cell lengths	71.326, 71.326, 220.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.600 - 1.906
R-factor	0.1933
Rwork	0.192
R-free	0.21830
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.152
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.611	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	43347
Completeness [%]		99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.02M proline, 0.1M Hepes pH 7.5, 10~7% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.02M proline, 0.1M Hepes pH 7.5, 10~7% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K