3RTK
Crystal structure of Cpn60.2 from Mycobacterium tuberculosis at 2.8A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-28 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.190, 111.980, 77.280 |
| Unit cell angles | 90.00, 95.50, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| Rwork | 0.217 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sjp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.530 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 12.530 | 2.870 |
| High resolution limit [Å] | 2.800 | 8.860 | 2.800 |
| Rmerge | 0.058 | ||
| Number of reflections | 23832 | ||
| <I/σ(I)> | 17.28 | 45.12 | 1.9 |
| Completeness [%] | 2.84 | 10.25 | |
| Redundancy | 3.6 | 3.42 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M HEPES, pH 7.5, 10% 2-propanol, 20% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
