3RT1
Maltodextarn bound activated state form of yeast glycogen synthase isoform 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 200 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 193.439, 205.328, 206.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.580 - 2.800 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.503 | |
| Number of reflections | 96658 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 293 | 0.1M Bis-Tris, 25% PEG 300, VAPOR DIFFUSION, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.2 |
