3RSZ
Maltodextran bound basal state conformation of yeast glycogen synthase isoform 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-15 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.557, 166.731, 121.136 |
| Unit cell angles | 90.00, 103.25, 90.00 |
Refinement procedure
| Resolution | 47.564 - 3.009 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3naz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.152 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.082 | 0.525 |
| Number of reflections | 73079 | |
| <I/σ(I)> | 13.2 | 2 |
| Completeness [%] | 94.7 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% PEG 3400, 0.1M Lithium Sulfate, 0.1M Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
