3RSP
STRUCTURE OF THE P93G VARIANT OF RIBONUCLEASE A
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-04-11 |
| Detector | SIEMENS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.790, 65.790, 66.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.185 * |
| Rwork | 0.170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rph |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.504 |
| Data reduction software | XCALIBRE |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.028 | 0.150 |
| Number of reflections | 22780 | |
| <I/σ(I)> | 20 | 3 |
| Completeness [%] | 93.0 | 93 |
| Redundancy | 2.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | 6UL DROPS COMPOSED OF 15MG/ML PROTEIN,50MM SODIUM ACETATE AT PH 6.0, 15% SATURATED AMMONIUM SULFATE AND 25% SATURATED SODIUM CHLORIDE WERE SUSPENDED OVER 0.5ML WELLS CONTAINING 100MM SODIUM ACETATE, PH 6.0, 30% SATURATED AMMONIUM SULFATE AND 50% SATURATED SODIUM CHLORIDE. TEMPERATURE: 20C., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 50 (mM) | |
| 2 | 1 | drop | protein | 15 (mg/ml) | |
| 3 | 1 | drop | ammonium sulfate | 15 (%(v/v)sat) | |
| 4 | 1 | drop | 25 (%(v/v)sat) | ||
| 5 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 30 (%(v/v)sat) | |
| 7 | 1 | reservoir | 50 (%(v/v)sat) |
