3RS5
H-Ras soaked in 55% dimethylformamide: 1 of 10 in MSCS set
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.531, 88.531, 134.948 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.221 - 1.680 |
| R-factor | 0.1644 |
| Rwork | 0.162 |
| R-free | 0.18190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.166 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 3.620 | 1.680 |
| Rmerge | 0.034 | 0.026 | 0.118 |
| Number of reflections | 23412 | ||
| <I/σ(I)> | 18 | ||
| Completeness [%] | 99.8 | 98.3 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | 20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K |
