3RRG
Ternary Structure of the large fragment of Taq DNA polymerase bound to an abasic site and a ddGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.279, 110.279, 90.966 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.752 - 2.300 |
| R-factor | 0.2003 |
| Rwork | 0.198 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lwl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (dev_704) |
| Refinement software | PHENIX ((phenix.refine: dev_704)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.146 | 0.914 |
| <I/σ(I)> | 13.69 | 1.52 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 9.96 | 9.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05 M Na cacodylate pH 6.5, 0.2 M NH4OAc, 0.01 M Mg(OAc)2, 28% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
