3RRA
Crystal structure of enolase PRK14017 (target EFI-500653) from Ralstonia pickettii 12J with magnesium bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-03-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 4 |
| Unit cell lengths | 117.339, 117.339, 113.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.20763 |
| Rwork | 0.206 |
| R-free | 0.26258 |
| Structure solution method | MR |
| Starting model (for MR) | 3rr1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.118 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.900 | |
| Number of reflections | 34611 | |
| <I/σ(I)> | 7.2 | 1.2 |
| Completeness [%] | 94.8 | 92.8 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1M TRIS-HCL, 200MM AMMONIUM ACETATE, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
