3RR2

Structure of a Cysteine synthase (O-Acetylserine Sulfhydrylase (OASS)) from Mycobacterium marinum ATCC BAA-535 / M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Detector technology	CCD
Collection date	2011-03-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	51.190, 51.190, 229.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.760 - 1.950
R-factor	0.1902
Rwork	0.188
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.275
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.760		2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.085	0.032	0.508
Number of reflections	23516	354	1682
<I/σ(I)>	19.29	48	4.05
Completeness [%]	99.9	98.3	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1