3RQG
Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.941, 116.159, 124.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.280 - 2.500 |
| R-factor | 0.24359 |
| Rwork | 0.241 |
| R-free | 0.29106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l8i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.985 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.111 | 0.984 |
| <I/σ(I)> | 18.8 | 2.436 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1-0.2 M potassium fluoride, 15-20% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
