3RQ4
Crystal structure of suppressor of variegation 4-20 homolog 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.680, 60.151, 126.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.17975 |
| Rwork | 0.178 |
| R-free | 0.22115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SUV420H1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.300 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.119 | 0.358 |
| Number of reflections | 25132 | |
| <I/σ(I)> | 26.6 | 2.55 |
| Completeness [%] | 98.7 | 83.3 |
| Redundancy | 8.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 12% PEG8K, 0.1MHepes, 12% Ethylene Glycol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
