3RPX

Crystal structure of complement component 1, q subcomponent binding protein, C1QBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97920
Spacegroup name	I 2 2 2
Unit cell lengths	115.680, 123.770, 137.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 - 2.650
R-factor	0.1965
Rwork	0.195
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p32
RMSD bond length	0.010
RMSD bond angle	1.080
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000		2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge	0.074	0.024	0.972
Number of reflections	28824	285	2097
<I/σ(I)>	18.46	58.1	2.2
Completeness [%]	99.6	75.4	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% peg1500, 0.2 M sodium chloride, 0.1 M HEPES, 5% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K