3RPU

Crystal structure of the MukE-MukF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0809
Spacegroup name	P 65 2 2
Unit cell lengths	149.890, 149.890, 738.551
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.977 - 3.600
R-factor	0.2202
Rwork	0.217
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3euh
RMSD bond length	0.021
RMSD bond angle	1.736
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	3.730
High resolution limit [Å]	3.600	7.620	3.600
Rmerge	0.104	0.040	0.529
Number of reflections	57367
<I/σ(I)>	9.7	21.86	1.4
Completeness [%]	97.3	92.9	99.7
Redundancy	3.5	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.78-0.9 M Na/K phosphate, 25 mM MgSO4 and 5% (v/v) MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K