3RP1
Crystal structure of Human LAIR-1 in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0810 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.117, 77.261, 64.940 |
| Unit cell angles | 90.00, 120.18, 90.00 |
Refinement procedure
| Resolution | 31.820 - 2.600 |
| R-factor | 0.2173 |
| Rwork | 0.211 |
| R-free | 0.29270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kgr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 12005 | |
| <I/σ(I)> | 17.8 | 4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 200mM calcium choloride, 28% PEG400, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
