3ROB
The crystal structure of a conserved protein from Planctomyces limnophilus DSM 3776
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 31 |
| Unit cell lengths | 68.098, 68.098, 124.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.487 - 1.480 |
| R-factor | 0.1733 |
| Rwork | 0.172 |
| R-free | 0.19370 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.084 | 0.565 |
| Number of reflections | 107855 | |
| <I/σ(I)> | 38 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M Tris:HCl, 15% (v/v) Ethanol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
