3RNX

Crystal Structure of Lysozyme in 30% ethanol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.242, 78.242, 36.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.419 - 1.856
R-factor	0.1765
Rwork	0.175
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ykx
RMSD bond length	0.007
RMSD bond angle	1.091
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.856	1.860
Rmerge	0.040	0.068
Number of reflections	10140
<I/σ(I)>	39.9	33.6
Completeness [%]	99.5	99.9
Redundancy	7.4	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	277	40 mM sodium acetate, 150mM sodium chloride, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K