3RN2
Structural Basis of Cytosolic DNA Recognition by Innate Immune Receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-10-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.560, 74.770, 65.520 |
| Unit cell angles | 90.00, 89.73, 90.00 |
Refinement procedure
| Resolution | 43.765 - 2.550 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | code 3RLO |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.544 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_723)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.050 | 0.617 |
| Number of reflections | 18538 | |
| <I/σ(I)> | 25.49 | 3.11 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.16 | 4.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8% PEG1000, 0.1 M KCl, 10 mM MgCl2, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
