3RMQ
Crystal structure of uncharacterized protein Svir_20580 from Saccharomonospora viridis (V71M mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 61 |
| Unit cell lengths | 79.225, 79.225, 47.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.932 - 1.850 |
| R-factor | 0.1639 |
| Rwork | 0.162 |
| R-free | 0.19080 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.095 | 0.646 |
| Number of reflections | 14408 | |
| <I/σ(I)> | 25.6 | 2.6 |
| Completeness [%] | 98.7 | 95.9 |
| Redundancy | 5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 1.8 M NaCl, 0.1 M Tris/HCl, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
